Temperature and Enzyme Activity
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Temperature and enzyme activity
Shobiga Peiris
Aim: To investigate the effect of temperature on enzyme activity.
Hypothesis: Enzyme activity is more effective at room temperature (37 degrees)
Materials:
Seven test tubes ( one test tube is the controlled test tube)
Six beakers (used as water baths)
Pineapple juice
30ml of milk
Stop watch
3 ice cubes
Water
Bunsen Burner
Gauze mat
Tripod
Thermometer
Test tube rack
Match Box
Risk assessment:
Wear goggles to protect your eyes when heating water
Wear gloves when handling hot water to protect your hands from burning.
Always stand to avoid spillage to the face or body.
Method:
Gather all required materials
Place 5ml of milk in each test tube
Add 3 drops of pineapple in each test tube.
Add 120ml of water in each of the six beakers (water baths)
Hold the thermometer around the middle of the water baths, (DONT NOT ALLOW THE THERMOETER TO TOUCH THE BOTTOM). Hold one thermometer in each separate water bath.
Allow the first water bath to reach the temperature of 27®c
Allow the second water bath to reach the temperature of 37®c (Room temperature)
Allow the third water bath to reach the temperature of 50®c
Allow the fourth water bath to reach the temperature of 70®c
Allow the fifth water bath to reach the temperature of 100®c
Place the 3 ice cubes in the water bath at the temperature of 0®c
Start the stopwatch and remove the test tube after 10 minutes in the water bath.
Place the test tubes in the rest tube rack.
Record for each individual test tube the results of the solution and see if it has varied or become more solidified.
Create a table of your observations
Repeat for reliability.