Essay About List Of Chemicals And Sulphuric Acid2

Methodology
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MethodologyChemicalsThere are some chemicals that have to be used during experimental work during the researchpalm oil empty fruit bunch convert to sugar. The list of chemicals as follows:-      Table 1.1 The list of chemicals used1Diluted sulphuric acid2Deionised water31% of dinitrosalicylic acid4Stock solution of anhydrous D-gulose at 10 g/L5Phenol6Sodium Sulphite7NaoH8Potassium sodium tartarateApparatusThere are some apparatus that have to used during the experimental work in this research palm oil empty fruit bunch convert to sugar. The list of the apparatus are as follow : Table 1.2 The list of apparatus used1Micropippete2U/Visible spectrophotometr3Autoclaved4Centrifuge5Waterbath6Centrifuge tubesMedia preparationThe EFB was collected at a nearby oil palm company, Felda Lepar Hilir 2, Kuantan, Pahang. After the collection of sample,  the EFB was washed with deionized water to remove dirt and debris That attached it and was cut into smaller pieces. For 3 consecutive days, the EFB was oven-dried at 80°C . By using a fibre grinder, the dried EFB were grounded to approximately 0.5mm ~ 10mm. The particle size was determine by using a sieve tray.EFB Pretreatment – Acid HydrolysisEFB (10 g ) was added into different concentration of sulphuric acid ( H2SO4) 1%, 2%, 4%, 6%and autoclaved at 130°C, pressure 10 psi for 1 hour. After  reaching the required reaction time, the reaction mixture was centrifuged for 10 minutes, 12 000 rpm and 10°C. Then, the solid was separated from the liquid  phase by filtration.1.5. Dinitrosalisalicylic acid (DNS)The reducing sugar are determined by using dinitrosalicylic Acid (DNS) by standard glucose