Essay About Applications Of Fluorescent Probes And Hydrolase Enzyme

Esterases Are Hydrolase Enzyme – Thesis – Madhushree Phalak
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Esterases Are Hydrolase Enzyme
Annex-V  PK- 1/ 1SVKM’S NMIMSSHOBHABEN PRATAPBHAI PATEL SCHOOL OF PHARMACY & TECHNOLOGY        MANAGEMENT, MUMBAISYNOPSIS                MINOR PROJECT – M.Pharm. +MBAAcademic Year- (2017-2018)Name of the Student: Ms. Madhushree PhalakProgramme and Specialization: M.Pharm. (Quality Assurance)+MBAName of the Guide: Dr. Prashant S. KharkarTitle: Applications of Fluorescent Probes for Esterase detection.Introduction:Esterases are hydrolase enzyme present in all the living organisms which can be used to provide information on the metabolic state of the bacterial cells. [1]Enzyme-activated, fluorescence probes are powerful tools for studying bacterial pathogens, including Mycobacterium tuberculosis. These small molecule probes are versatile tools as they target specific enzyme classes in complex mixtures of living organisms. [2]Detecting and characterizing these esterases has been difficult because most of them form inclusion bodies in heterologous hosts , fluorogenic enzyme probes can directly detect enzyme activities in intact organisms or in protein gel-resolved lysates without the need for isolated enzyme. [3]Since the probe excites above 600nm, cellular autofluorescence and light scattering is minimal in this region of the spectrum, giving the probe superior fluorescent properties for imaging in living systems. [4] A variety of enzyme-targeted probes have been used to better understand the bacterial strains and substrate analogues have been employed to annotate the genome, identify therapeutic   targets,  and characterize enzymatic activity. [4]Objectives: To carry out enzyme kinetic evaluation of the prepared Fluorogenic Probes, using various sources of esterases. With the prepared fluorogenic probes, we look forward to developing assays that can stratify bacterial strains, annotate enzyme function, and track specific esterase activities during different stages of bacterial growth. Plan of Work:Synthesis of the Fluorescence Probes from the acquired materials.Characterization of Fluorescence Probes Spectral Characteristics will be determined (Absorbance and Emission spectra’s , Extinction co-efficients ,Quantum yields )Assessment of the hydrolytic stability of the prepared fluorescence probes.Examination of the sensitivity of the probes by determining the PLE detection limits for the prepared probes.Evaluation of the probes for enzyme kinetics. Conducting gel based assay for the probes.Time Line of the Project:Sr.Nature of workDuration in weeksNo.1.Literature and patent search and Review Article.12.Listing and procurement of materials23.Synthesis of the Fluorescence Probes from the acquired materials4.Characterization of Fluorescence Probes5.Assessment of the hydrolytic stability of the prepared fluorescence probes        6.Examination of the sensitivity of the probes by determining the PLE detection limits for the prepared probes7.Evaluation of the probes for enzyme kinetics. 8.Conducting gel based assay for the probes.9.Patent/Research Publication110.Thesis submission1Total24 Weeks

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“Esterases Are Hydrolase Enzyme” EssaysForStudent.com. 02 2019. 2019. 02 2019 < "Esterases Are Hydrolase Enzyme." EssaysForStudent.com. EssaysForStudent.com, 02 2019. Web. 02 2019. < "Esterases Are Hydrolase Enzyme." EssaysForStudent.com. 02, 2019. Accessed 02, 2019. Essay Preview By: Madhushree Phalak Submitted: February 4, 2019 Essay Length: 707 Words / 3 Pages Paper type: Thesis Views: 205 Report this essay Tweet Related Essays Determining Optimum Environmental and Chemical Effects on Enzyme Kinetics Abstract Enzymes, fundamental proteins that catalyze reactions by lowering activation energy, operate under kinetic principle and can react slower or faster with different ph and 1,127 Words  |  5 Pages Enzymes Essay Enzymes Enzymes are the sparks that start the essential chemical reactions our bodies need to live. They are necessary for digesting food, for stimulating the 722 Words  |  3 Pages Importance of Digestive Enzymes ENZYME TIME (posted 9-7) Written by: Len Davis Creatine. Check. Arginine. Check. Protein powder. Check. Enzymes. Che . . . wait, what was that last 444 Words  |  2 Pages Enzyme Examination of the Effects of Inhibitory and Non-Inhibitory Competition, Enzyme-Substrate Concentration, Along with Varying Temperature and pH-Balanced Environments on the Enzyme-Catalyzed Reaction of pNPP Abstract: 1,882 Words  |  8 Pages Similar Topics Factors Effecting Enzymes Factors Affecting Rate Enzyme Activity Get Access to 89,000+ Essays and Term Papers Join 209,000+ Other Students High Quality Essays and Documents Sign up © 2008–2020 EssaysForStudent.comFree Essays, Book Reports, Term Papers and Research Papers Essays Sign up Sign in Contact us Site Map Privacy Policy Terms of Service Facebook Twitter