Micro Lad Report Media Broth
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Today in lab we made a broth media using deionized water (DH2O2) and chemical powder. We used a specific math formula, which help determine water to media ratio. . We then heated the broth to dissolve all the powder, evenly distributed the broth among 6 tubes, and in the autoclave to sterilize it to provide the optimal environment for our bacteria to grow in the future.
Methods
* Turn on hot plate
* Calculate amount of powder needed using the formula below
Total DH2O2 X Grams of media needed = Media per tube = amount of media need
needed
Per 1 Liter DH2O2
1 liter DH2O2
(1000ml)
DH2O2= 48 ml- for all 6 tubes (8ml per tube)
Media = 23 grams per liter
48 X 23g =1.104g (or 1.1g)
1000ml
* Weigh Boat (bowl to weigh powder in)before adding powder . Boat = 1.9g
* Place scale to 3g (1.9g boat + 1.1g powder = 3g). Add powder using metal spatula until scale is balanced
* Add 45ml of DH2O2 into a flask, using 5 ml pipette (9 times)
* Pour powder into flask.
* With the remaining 3 ml of DH2O2, using the 1ml pipette, put 1 ml into boat at a time making sure to get all powder off the boat.
* Pour mixture (powder and 3ml DH2O2) into flask with the other media mixture.
* Stir broth with a glass stirrer, making sure to scrap the bottom of the flask
* Place flask on hot plate.
* Let boil/cook for a few minute until powder is completely dissolved.
* Keep a close eye so broth does not boil over.
* Remove flask from hot plate with gloves.
* Using the 5ml and 1ml pipette dispense 8ml of broth into each of the 6 tubes.
* Label each tube with autoclave tape ad write name, date and substance in tubes.
* Place the 6 tubes into a rack and then into the autoclave machine.
Conclusions