Color Reactions of Carbohydrates – Lab Report – 151336984
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Color Reactions of Carbohydrates
Color Reactions For carbohydratesTest RxExpected ResultComponent Responsible forVRSugars which gives (+) resultGeneral TestMolischDetect compounds other than CHOα- NaphtholReacts w/ cyclic aldehydesConc. H2SO4[pic 1]Furfural           Naphtholstrong acid(conc. H2SO4)2 layers will formsugar hydrolyze to become monosaccharide:  removed H2O  → formedfurfural or Hydroxymethylfurfural→condensed with (αnephthol )to form a violet ring(Furfuryldiphenylmethane orFurfurylDinaphthylFurfuralproduced from pentoses/pentosansHydroxymethylfurfuralproduced from hexoses and hexosansArabinose ,Cellulose, Fructose,Galactose,Glucose, Inulin ,LatoseMaltose, starch ,SucroseDifferentiating Test for aldoses  and KetosesSeliwanoffRESORCINOLConcHCl:dehydrate sugars[pic 2]Cherry red (ketoses)& yellow to faint pink color(aldohexoses)Ketoses( react faster)dehydrated:furfural derivatives(Hydroxymethylfurfural)→resorcinol: redcomplexAldosesFructose- KetoseGlucose- aldoseInulin (red)-ketoseSucrose-bothPentoses (Green color)-presence:resorcinoleg. Arabinose, xyloseBialsQualitative test for pentoses& nucleotides w/ pentosesOrcinolFe3+ ionsBlu-green ColorFurfural  (pentoses)Heated with conc. HClReacts with orcinolArabinose, XyloseBarfoed’s testCupric acetate Glacial acetic acidbrick  red pptCuprous OxideGlucoseTest for Reducing Sugar ( R – CHO  + 2Cu2+ +4OH→R – COOH + Cu2O + 2H2O)Benedict TestSodium CitrateNa3C6H5O7Insoluble brick  red pptCuprous Oxide (Cu2O)Fructose ,Glucose , Maltose &LactoseFehilngs TestBlusol’n indicates no reducing propertyA: CuSO4B: Rochelle salt( Sodium Potassium tartrate)Insoluble brick  red pptCuprous Oxide (Cu2O)Fructose ,Glucose , Maltose &Lactose“Tollen’s Silver Mirror TestAmmonium Hydroxide (ammonia silver Nitrate)1% Silver NitrateSilver Metal (Ag)GlucoseFerric Cyanide TestYellow colorAldoses or ketosesPhenylhydrazine Test (Osazone Formation)Orange or yellow orange (2,4) DinitrophenylhydrazineCrystallize :seen under low power objective microscopeGlucose(needle)  , fructose, (needle) maltose (sunflower), lactose (powdered puff )Specific Test for SugarsIndole3-acetic acidPresence of acid catalystIntence purple color (40 C- 50C)Fructose& large amount of glucoseIodine Test for starch•        This is a test for polysaccharides• Dextrins- Amylo, Eryhthro and Achrodextrins, formed as intermediates during hydrolysis of starch give violet, red and no color with iodine respectively.Deep blue black color →Reddish brownYellow-orange – negative. Purple-black -positive.Amylose – deep blue starch-iodo complex Amylopectin/glycogen – blue violet ↑amt :Reddish violetGlucose in the form of starchMucic Acid for GalactoseMonosaccarides by nitric acid yields sol. Dicarboxylic acids.InsolMucic acid: due to hydrolysis of glycosidic linkage b/w glucose &galactosegalactoseDehydration
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By: 151336984
Submitted: October 31, 2016
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