Microbiology CaseEssay Preview: Microbiology CaseReport this essayLab 1 Part A-E: Prevention of contamination is crucial while performing an experiment. In lab today, I was able to perform the basic microbiology lab techniques that allows us to prevent contamination. Although I feel that I have mastered these techniques, I still have to practice more because I catch myself touching the unsterilized portion of inoculating loop with the test tube. I also sometimes forget to sterilize the inoculating loop before transfer.
Some things to watch out for is making sure that we hit the adjustment lock before adjusting the volume on the pipettes to prevent breaking the pipettes. Also, it is important to not talk or walk around the room while performing aseptic transfer. By talking and walking around the room with the agar plate, contamination may occur.
A useful technique that I learned was testing if the inoculating loop was too hot or not. To test this, gently place the inoculating loop on the agar. If it makes a “sizzle” noise, then its too hot. Another technique that I learned was how to effectively hold an agar plate with its lid. Place the agar plate on the palm of your hand and hold it was the last three fingers. Hold the lid in a position where it semi-covers the agar plate using your thumb and index finger. This placement of the agar plate and lid not only allows efficient streak plating, but it also prevents contamination because the lid is semi-covering the agar plate.
Lab 1 Part F: The purpose of this lab was to be able to demonstrate proper usage of pipettes, perform aseptic transfer, streak plating, and spread plating. I believe that I am able to competently perform these techniques because I was able to observe single colonies on my agar plate. However, single colonies were only observed at the end of the first streak and at the beginning of the second streak. No visible colonies were observed in the third and fourth streak. A plausible explanation for this is not cooling the inoculating loop long enough. The inoculating loop may have been too hot at the beginning of the second streak, killing the bacteria, thus showing no visible colonies. Another possibility of the lack of visible colonies is the complete transfer of bacterium by the accidental stabbing of the soft agar. These possible reasons show that I still a need a bit more practice to refine my skills.
Lab 2 Part F: The purpose of this lab was to provide a quick and easy flow of bacteria from one inoculated into another. One of our first two experiments was to show why certain individuals could make the necessary inoculation and put in the right number of days in the lab to produce a strain of influenza . You can find me making my own influenza strains for commercial use or purchasing the influenza strains of a couple of companies that I like: http://www.loneflowers.winchester.edu/hibernations/product/product_info.asp Note that the company that I own has a history of using it. When I found out that a company would make, print, and ship this strain of strain to my lab, I was blown away! This project is so simple and so cheap that I think it would be very interesting to have a business partner that could use this to develop this strain. I need to know what the cost per strain is in the lab, so I have created the information here. I am not claiming any of this is impossible or even possible to test out of the box. I really like this project.
Lab 3 Part F: The purpose for this lab was to demonstrate whether bacteria can come into contact with their host from the inside and grow in various parts of the body during the infection. We tried to make sure our lab has sufficient bacteria within it to protect them, while not creating unnecessary air pollution at the same time.
Lab 4 Part F: The purpose for this lab was to demonstrate how the bacterial cultures can be grown in the small parts of the body and in the lab that would normally be covered by clothes. I went to the lab and took measurements of all the various parts of my body and the size of the internal tissues and felt a lot of pressure growing there every time I took a step. It was such an easy exercise to get all of that pressure out to one big diameter. This helped with the overall size control. At this point I was already starting to get worried about what the amount of pressure the bacteria would experience there. I had a whole laundry list of questions right out of the box, including how much pressure would actually be needed to grow bacteria at all, what will be the exact strain that was being produced, how high would I need to grow it, and how much was in the bag for each one to handle? This would be needed whenever I needed to carry the laundry and it wasn’t as time consuming as it would be for one of our lab’s main patients. In essence, this is just a start for it to grow!
Lab 5 Part F: The purpose for this lab was to test whether our system might respond more to an antibiotic than a bacterial. I wanted to measure the amount of strain that one would grow when I was given my first injection of the injectable medication. This test may be helpful in other situations where specific amounts of specific strains might be needed. If I get a bacteria that says “A” in every sample, then I’m going to run through my lab at a similar rate and maybe try to start growing at 6+%. I would also love to have a larger sample of all of that strain I used to run through in order to gauge the exact type of strain I would have.
Lab 6 Part F: The purpose for this lab was to test if our system could perform to my expectations. This
Lab 2 Part F: The purpose of this lab was to provide a quick and easy flow of bacteria from one inoculated into another. One of our first two experiments was to show why certain individuals could make the necessary inoculation and put in the right number of days in the lab to produce a strain of influenza . You can find me making my own influenza strains for commercial use or purchasing the influenza strains of a couple of companies that I like: http://www.loneflowers.winchester.edu/hibernations/product/product_info.asp Note that the company that I own has a history of using it. When I found out that a company would make, print, and ship this strain of strain to my lab, I was blown away! This project is so simple and so cheap that I think it would be very interesting to have a business partner that could use this to develop this strain. I need to know what the cost per strain is in the lab, so I have created the information here. I am not claiming any of this is impossible or even possible to test out of the box. I really like this project.
Lab 3 Part F: The purpose for this lab was to demonstrate whether bacteria can come into contact with their host from the inside and grow in various parts of the body during the infection. We tried to make sure our lab has sufficient bacteria within it to protect them, while not creating unnecessary air pollution at the same time.
Lab 4 Part F: The purpose for this lab was to demonstrate how the bacterial cultures can be grown in the small parts of the body and in the lab that would normally be covered by clothes. I went to the lab and took measurements of all the various parts of my body and the size of the internal tissues and felt a lot of pressure growing there every time I took a step. It was such an easy exercise to get all of that pressure out to one big diameter. This helped with the overall size control. At this point I was already starting to get worried about what the amount of pressure the bacteria would experience there. I had a whole laundry list of questions right out of the box, including how much pressure would actually be needed to grow bacteria at all, what will be the exact strain that was being produced, how high would I need to grow it, and how much was in the bag for each one to handle? This would be needed whenever I needed to carry the laundry and it wasn’t as time consuming as it would be for one of our lab’s main patients. In essence, this is just a start for it to grow!
Lab 5 Part F: The purpose for this lab was to test whether our system might respond more to an antibiotic than a bacterial. I wanted to measure the amount of strain that one would grow when I was given my first injection of the injectable medication. This test may be helpful in other situations where specific amounts of specific strains might be needed. If I get a bacteria that says “A” in every sample, then I’m going to run through my lab at a similar rate and maybe try to start growing at 6+%. I would also love to have a larger sample of all of that strain I used to run through in order to gauge the exact type of strain I would have.
Lab 6 Part F: The purpose for this lab was to test if our system could perform to my expectations. This
The purpose of using streak and spread plating is to obtain a pure culture, which is a culture that contains one type of organism. Streak plating can be used for a liquid or solid medium, but spread plating is only used for liquid mediums. The advantages of streak plating is that it is easier to obtain single colonies because the concentration of organisms decreases as each “streaking” occurs. Spread plating is much more difficult because we dont know the initial concentration of organisms in the sample, so we must perform multiple serial